Device

Part:BBa_K1742011:Design

Designed by: Mónica Victoria Gutiérrez Salazar   Group: iGEM15_Valencia_UPV   (2015-09-07)


35S:LacIBDBD-DronpaK:T35S - 35S:DronpaN-VP16:T35S


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2317
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 66


Design Notes

Change the aminoacid.


Source

Dronpa(145K) is synthetized. Dronpa(145N) was obtained by PCR, changing 145K by 145N.

References